Based on 454 pyrosequencing of 16S rRNA genes, the identified genera of sulfate-reducing bacteria (SRB) included Desulfovibrio, Desulfomicrobium and Desulfococcus. This study showed that sulfate-rich AMD can be effectively treated by integrating hydrotalcite precipitation and a biological sulfate reducing FBR.Microplastic pollution is an important issue for environmental management as their ubiquity in marine and freshwaters has been confirmed. Pollution sources are key to understanding how microplastics travel from land to open oceans. Given that information regarding microplastic transport from diffuse sources is limiting, we conducted a study on roadside dust from rural and urban Victoria, Australia, over two seasons. Any deposited fugitive dust and particulate matter may also be present in our samples. The average microplastic abundance over two seasons ranged from 20.6 to 529.3 items/kg (dry weight based), with a predominant portion of fibers and items less than 1 mm. Polyester and polypropylene were the dominant polymer types (26%) while cotton and cellulose were the most common non-plastic items (27%). Sampling sites displayed consistent microplastic abundances over time and shared similar patterns in size, shape and polymer composition. Multiple correlation and principal component analysis suggest that urbanization and rainfall are important influences to roadside microplastic accumulation. The observed microplastic hotspots were generally located within close vicinity of areas where urban intensive land use and regional population sizes are high. Microplastics accumulated on roads and road verges during periods of dry weather and were flushed away during heavy storms while the corresponding trigger value was unknown. Monitoring roadside dust can be considered as an initial and cost-effective screening of microplastic pollution in urban areas. Further efforts should be made to optimize the methodologies and we advocate prolonged sampling schemes for roadside dust monitoring.Climate change is leading to a gradual increase in the ocean temperature, which can cause physiological and biochemical impairments in aquatic organisms. Along with the environmental changes, the presence of emerging pollutants such as titanium dioxide (TiO2) in marine coastal systems has also been a topic of concern, especially considering the interactive effects that both factors may present to inhabiting organisms. In the present study, it has been assessed the effects of the presence in water of particles of rutile, the most common polymorph of TiO2, in Mytilus galloprovincialis, under actual and predicted warming conditions. Organisms were exposed to different concentrations of rutile (0, 5, 50, 100 μg/L) at control (18 ± 1.0 °C) and increased (22 ± 1.0 °C) temperatures. Histopathological and biochemical changes were evaluated in mussels after 28 days of exposure. Histopathological examination revealed similar alterations on mussels' gills and digestive glands with increasing rutile concentrations at both temperatures. Biochemical markers showed that contaminated mussels have an unchanged metabolic capacity at 18 °C, which increased at 22 °C. Although antioxidant defences were activated in contaminated organisms at 22 °C, cellular damage was still observed. Overall, our findings showed that histopathological impacts occurred after rutile exposure regardless of the temperature, while biochemical alterations were only significantly noticeable when temperature was enhanced to 22 °C. Thus, this study demonstrated that temperature rise may significantly enhance the sensitivity of bivalves towards emerging pollutants.The landfill sludge in storage reservoirs needs to be dewatered and disposed of for environmental and engineering purposes. The key factors are the high organic matter content and low permeability. Chemical conditioning is considered an efficient method for adjusting the properties of sludge. In this paper, two typical chemical agents, FeCl3 and a Fenton reagent with different additive amounts, are studied and compared for dewatering and consolidation purposes. Compression experiments and consolidation experiments are compared, and the coefficient of compressibility and compression index are obtained and compared. Then, the sludge permeability, grain size distribution variations, specific resistance to filtration (SRF) and morphology observations are considered to analyse the treatment mechanism. The results indicate that the properties of landfill sludge will change as the curing time increases. FeCl3 and Fenton are both effective in improving the consolidation and permeability properties of sludge. For the conditioning process, the optimum FeCl3 content is 20%, and the process is dominated by coagulation if FeCl3 is less than 20%; otherwise, it is dominated by hydrolysis. For the Fenton reagent, the optimum Fe2+ content and H2O2 content are 4% and 12%, respectively. The depolymerization effect of the Fenton reagent leads to the oxidation and recombination of the polar group on extracellular polymeric substances (EPSs). The results can be used to explain the conditioning mechanism of the effective agents of FeCl3 and Fenton and compare the corresponding consolidation properties. The consolidation characteristics provide a reference for further application of vacuum preloading in the sludge disposal process.There is limited research on the effects of gut microbiota on bioaccessibility of heavy metals in wheat grains. In this study, bioaccessibility of heavy metals (Cu, Cd, Pb, and Zn) in wheat was determined to elucidate transfer characteristics in the soil-grain-human systems near two large-scale mining areas in Shandong Province, North China using the physiologically-based extraction test (PBET) in combination with a simulator of human intestinal microbial ecosystems (SHIME). https://www.selleckchem.com/products/fdi-6.html The results showed the bioconcentration factors (BCFs) of Cu, Cd, Pb, and Zn were 0.123-0.327, 0.188-0.478, 0.019-0.099, and 0.262-0.825, respectively. Significant and positive correlations were observed between heavy metals in soils and wheat grains. In the simulated colon phase, bioaccessibility of Cd and Zn significantly decreased to 7.81% and 8.81%, respectively, being 53% and 64% of that in the simulated small intestinal phase. However, bioaccessibility of Pb showed an obvious escalating trend, being 2.4 times higher than that of intestinal incubation.